rabbit anti p irf7 polyclonal antibody  (Cell Signaling Technology Inc)

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication

doi: 10.3389/fcimb.2024.1529159

Figure Lengend Snippet: List of primers used in RT-qPCR.

Article Snippet: Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China).

Techniques: Sequencing

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication

doi: 10.3389/fcimb.2024.1529159

Figure Lengend Snippet: IBDV infection can suppress the expression of IRF7. IBDV viral load, relative expression levels of the IRF7 and IFN-β gene (detected by RT-qPCR) in DF-1 cells infected with 1 MOI vvIBDV NN1172 (A) or attenuated IBDV B87 (B) at different time points. (C) IBDV viral load, relative expression levels of the IRF7 and IFN-β gene in DF-1 cells infected with 0.5, 1, or 1.5 MOI vvIBDV at different time points. (D-F) The IRF7 and VP2 protein levels in DF-1 cells (detected by western blot) infected with 0.5, 1, 1.5 MOI vvIBDV at different time point. (G) The protein levels of IFN-β at different time points after varying titers of IBDV infection. Three independent experiments were conducted, and the data are presented as the mean ± standard deviation of three replicates from a representative experiment. *p < 0.05; **p < 0.01.

Article Snippet: Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China).

Techniques: Infection, Expressing, Quantitative RT-PCR, Western Blot, Standard Deviation

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication

doi: 10.3389/fcimb.2024.1529159

Figure Lengend Snippet: Overexpression of IRF7 inhibits IBDV replication. (A) After transfecting DF-1 cells with the pcDNA3.1-His-IRF7 plasmid for 6 hours, cells were infected with 1 MOI vvIBDV. Samples were collected at various time points, and western blot was used to measure the expression levels of IRF7 and IBDV VP2 proteins. Alternatively, relative intensities of VP2 were normalized to β-actin. (B) RT-qPCR was employed to measure the IBDV viral load at various time points. Three independent experiments were conducted, and the data are presented as the mean ± standard deviation of three replicates from a representative experiment. *p < 0.05; **p < 0.01.

Article Snippet: Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China).

Techniques: Over Expression, Plasmid Preparation, Infection, Western Blot, Expressing, Quantitative RT-PCR, Standard Deviation

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication

doi: 10.3389/fcimb.2024.1529159

Figure Lengend Snippet: Knocking down IRF7 promotes IBDV replication. (A) DF-1 cells were transfected with siRNA targeting IRF7 for 6 hours, followed by infection with 1 MOI vvIBDV. Samples were collected at various time points, and western blot was used to measure the expression levels of IRF7 and IBDV VP2 proteins. Alternatively, relative intensities of IRF7 and VP2 were normalized to β-actin, respectively. (B) RT-qPCR was employed to measure the IBDV viral load at various time points. Three independent experiments were conducted, and the data are presented as the mean ± standard deviation of three replicates from a representative experiment. *p < 0.05; **p < 0.01.

Article Snippet: Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China).

Techniques: Transfection, Infection, Western Blot, Expressing, Quantitative RT-PCR, Standard Deviation

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication

doi: 10.3389/fcimb.2024.1529159

Figure Lengend Snippet: Overexpression of IRF7 couldn’t inhibit IRF7 degradation in vvIBDV-infected cells. (A) DF-1 cells were transfected with pcDNA3.1-His-IRF7 and infected with 1 MOI vvIBDV. Samples were collected at 6, 9, 12, 18, and 24 hpi, and the expression of IRF7 protein was detected using western blot. Alternatively, relative intensities of IRF7 were normalized to β-actin. (B) DF-1 cells were transfected with pcDNA3.1-His-IRF7 and infected with 1 MOI attenuated IBDV. Samples were collected at 12, 18, 24, and 36 hpi, and the expression of IRF7 protein was detected using western blot. Alternatively, relative intensities of IRF7 were normalized to β-actin. Three independent experiments were conducted, and the data are presented as the mean ± standard deviation of three replicates from a representative experiment. **p < 0.01.

Article Snippet: Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China).

Techniques: Over Expression, Infection, Transfection, Expressing, Western Blot, Standard Deviation

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication

doi: 10.3389/fcimb.2024.1529159

Figure Lengend Snippet: IBDV affects the expression of IRF7 through the proteasomal pathway. In the control group, cells were treated with DMSO, while in the experimental group, cells were treated with different inhibitors (PYR-41, Wortmannin, MG132) after being infected with vvIBDV. Samples were then collected for western blot analysis to measure the expression levels of IRF7 and VP2 proteins. Alternatively, relative intensities of IRF7 and VP2 were normalized to β-actin. Three independent experiments were conducted, and the data are presented as the mean ± standard deviation of three replicates from a representative experiment. **p < 0.01.

Article Snippet: Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China).

Techniques: Expressing, Control, Infection, Western Blot, Standard Deviation

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication

doi: 10.3389/fcimb.2024.1529159

Figure Lengend Snippet: Interaction between IBDV VP3 and IRF7. (A) After co-transfecting DF-1 cells with Flag-VP3 and His-IRF7, we harvested samples 48 hours later. Cells were lysed and immunoprecipitation was performed using Flag-tag antibodies, followed by Western blot detection. (B) DF-1 cells transfected with p3×FLAG-CMV-14-VP3 and pcDNA3.1-His-IRF7 were subjected to immunofluorescence staining with anti-FLAG rabbit mAb and anti-His mouse mAb.

Article Snippet: Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China).

Techniques: Immunoprecipitation, FLAG-tag, Western Blot, Transfection, Immunofluorescence, Staining

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication

doi: 10.3389/fcimb.2024.1529159

Figure Lengend Snippet: Overexpression of IBDV VP3 inhibits IRF7 expression. (A) DF-1 cells were transfected with the p3×FLAG-CMV14-VP3 plasmid for 6 hours and then stimulated with polyI:C. RT-qPCR was used to detect the gene expression levels of IRF7 and IFN-β at various time points post-infection. (B) DF-1 cells were transfected with the p3×FLAG-CMV14-VP3 plasmid for 6 hours and then stimulated with polyI:C for 24 h Western blot analysis was performed to detect the protein expression levels of IRF7 and Flag-VP3. Alternatively, relative intensities of IRF7 were normalized to β-actin. Three independent experiments were performed, and the data are presented as the mean ± standard deviation of three replicates from a representative experiment. *p<0.05; **p<0.01.

Article Snippet: Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China).

Techniques: Over Expression, Expressing, Transfection, Plasmid Preparation, Quantitative RT-PCR, Infection, Western Blot, Standard Deviation

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication

doi: 10.3389/fcimb.2024.1529159

Figure Lengend Snippet: IBDV VP3 promote IRF7 degradation through the proteasome pathway. After transfecting DF-1 cells with the p3×FLAG-CMV14-VP3 plasmid for 6 hours, cells were treated with inhibitor: PYR-41, Wortmannin, or MG132, respectively. Samples were collected 24 h after polyI:C treatment and the IRF7 and VP2 protein levels were analyzed using Western blot. Alternatively, relative intensities of IRF7 were normalized to β-actin. Three independent experiments were conducted, and the data are presented as the mean ± standard deviation of three replicates from a representative experiment. **p < 0.01.

Article Snippet: Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China).

Techniques: Plasmid Preparation, Western Blot, Standard Deviation

Journal: Frontiers in Cell and Developmental Biology

Article Title: Genome-wide analysis reveals the MORC3-mediated repression of PD-L1 expression in head and neck cancer

doi: 10.3389/fcell.2024.1410130

Figure Lengend Snippet: MORC3 represses the expression of many IFN-associated target genes. (A) The expression levels of IFN-associated target genes MX2, IFIT1, IRF7, IRF9, IFITM3, IFITM1, IFI44, and IFIH1 in CAL 27 cells with or without MORC3 knockdown were analyzed by qRT-PCR. (B) The expression levels of IFN-associated target genes MX2, IFIT1, IRF7, IRF9, IFITM3, IFITM1, and IFI44 in CAL 27 cells with or without MORC3 overexpression were analyzed by qRT-PCR. (C) The correlation between the expression levels of MORC3 and IFN-associated target genes in single cells of head and neck cancer were evaluated by reanalyzing the expression data of GSE10332 in the database of CancerSEA. *, p < 0.05.

Article Snippet: The membranes were blocked with 5% skimmed milk and incubated overnight at 4°C with following antibodies: mouse monoclonal anti-MORC3 antibody (Santa Cruz Biotechnology, United States), rabbit monoclonal anti- interferon induced protein with tetratricopeptide repeats 2 (IFIT2) antibody (abcam, United States), rabbit monoclonal anti-interferon induced transmembrane protein 3 (IFITM3) antibody (Cell Signaling Technology, United States), rabbit monoclonal anti-interferon induced protein with tetratricopeptide repeats 1 (IFIT1) antibody (Cell Signaling Technology, United States), rabbit monoclonal anti-cyclin D1 (CCND1) (ABclonal, China), rabbit monoclonal anti-cyclinD2 (CCND2) (ABclonal, China), rabbit polyclonal anti-JUN (ABclonal, China), rabbit polyclonal anti-interferon regulatory factor 7 (IRF7) (Proteintech, China), rabbit polyclonal anti-PD-L1 antibody (Proteintech, China), rabbit polyclonal anti-DExD/H-Box Helicase 60 (DDX60) antibody (Proteintech, China), and mouse anti-β-actin (Abmart, China).

Techniques: Expressing, Knockdown, Quantitative RT-PCR, Over Expression

Journal: Veterinary Research

Article Title: DHAV 3CD targets IRF7 and RIG-I proteins to block the type I interferon upstream signaling pathway

doi: 10.1186/s13567-023-01134-4

Figure Lengend Snippet: All primer sequences used in this experiment

Article Snippet: A mouse anti-Flag monoclonal antibody (Cat: M185-3 S) and a mouse anti-HA monoclonal antibody (Cat: M132-3) were purchased from Medical & Biological Laboratories Co., Ltd. A rabbit anti-duck IRF7 polyclonal antibody, a HRP-conjugated goat anti-mouse IgG heavy chain antibody (Cat: AS064), and a HRP-conjugated goat anti-mouse IgG light chain antibody (Cat: AS062) were prepared by ABclonal Technology Co., Ltd. A rabbit anti-beta (β)-actin antibody (Cat: 20536-1-AP) was obtained from Proteintech Co., Ltd. A rabbit anti-HA monoclonal antibody (Cat: AF2305), a mouse IgG antibody (Cat: A7028), and a HRP-conjugated goat anti-mouse IgG (Cat: A0216) were purchased from Beyotime Co., Ltd. A rabbit anti-Histone H3 (Cat: TA6358), a mouse anti-Myc (Cat: T62076M), and a mouse anti-beta (β)-tubulin monoclonal antibody (Cat: T63017-2) were purchased from Abmart Co., Ltd. A rabbit anti-VP3 antibody was prepared in our laboratory [ ].

Techniques: Sequencing

Journal: Veterinary Research

Article Title: DHAV 3CD targets IRF7 and RIG-I proteins to block the type I interferon upstream signaling pathway

doi: 10.1186/s13567-023-01134-4

Figure Lengend Snippet: The 3CD protein interacts with IRF7 protein and inhibits the expression of IRF7 protein. A pCAGGS-3CD-HA and pCAGGS-IRF7-Flag were co-transfected or both were separately co-transfected with pCAGGS into 24-well plates (transfection ratio of 1:1), and cell samples were collected 36 h after transfection for indirect immunofluorescence experiments to observe the intracellular localization of the 3CD protein and IRF7 protein. B Cells were grown in 6-well plates and each well was transfected with a total of 1 µg pCAGGS-3CD-HA and 1 µg pCAGGS-IRF7-Flag. Cells were lysed 36 h after transfection, and lysates were immunoprecipitated with mouse anti-Flag or mouse IgG antibodies and subjected to Western blotting. C 0.4 µg pCAGGS-IRF7-Flag was co-transfected into 12-well plates with 0.6 µg pCAGGS-3CD-HA, pCAGGS-3D-HA, pCMV-3 C-HA or empty, respectively, and cell samples were collected 36 h after transfection to detect the expression of IRF7 protein. D Similar transfection and quantitative PCR experiments were performed to analyze the effect of 3CD protein on Poly(I:C)-induced IRF7 mRNA levels as described in Figure A. * P < 0.05, ** P < 0.01, *** P < 0.001, compared with the control group. E DEFs were grown in 12-well plates, and different doses of 3CD-HA expression plasmids were transferred into the cells (the amount transfected into each group was 1 µg, and pCAGGS was used to supplement the insufficient group). The expression of 3CD protein and endogenous IRF7 protein was detected by Western blotting at 36 h. F DEFs were grown in 12-well plates, and 0.4 µg of pCAGGS-IRF7-Flag was co-transfected with different doses of pCAGGS-3CD-HA (the amount transfected into each group was 1 µg, and pCAGGS was used to supplement the insufficient group). 36 h after transfection, cell samples were collected, and the protein expressions of 3CD and IRF7 were detected.

Article Snippet: A mouse anti-Flag monoclonal antibody (Cat: M185-3 S) and a mouse anti-HA monoclonal antibody (Cat: M132-3) were purchased from Medical & Biological Laboratories Co., Ltd. A rabbit anti-duck IRF7 polyclonal antibody, a HRP-conjugated goat anti-mouse IgG heavy chain antibody (Cat: AS064), and a HRP-conjugated goat anti-mouse IgG light chain antibody (Cat: AS062) were prepared by ABclonal Technology Co., Ltd. A rabbit anti-beta (β)-actin antibody (Cat: 20536-1-AP) was obtained from Proteintech Co., Ltd. A rabbit anti-HA monoclonal antibody (Cat: AF2305), a mouse IgG antibody (Cat: A7028), and a HRP-conjugated goat anti-mouse IgG (Cat: A0216) were purchased from Beyotime Co., Ltd. A rabbit anti-Histone H3 (Cat: TA6358), a mouse anti-Myc (Cat: T62076M), and a mouse anti-beta (β)-tubulin monoclonal antibody (Cat: T63017-2) were purchased from Abmart Co., Ltd. A rabbit anti-VP3 antibody was prepared in our laboratory [ ].

Techniques: Expressing, Transfection, Immunofluorescence, Immunoprecipitation, Western Blot, Real-time Polymerase Chain Reaction, Control

Journal: Veterinary Research

Article Title: DHAV 3CD targets IRF7 and RIG-I proteins to block the type I interferon upstream signaling pathway

doi: 10.1186/s13567-023-01134-4

Figure Lengend Snippet: Effect of the 3CD protein on the nuclear translocation of the IRF7 protein. A Three experimental groups were set up, both groups I and II were co-transfected with 0.3 µg pCAGGS-IRF7-Flag and 0.5 µg pCAGGS; the third group was transfected with 0.3 µg pCAGGS-IRF7-Flag and 0.5 µg pCAGGS-3CD-HA, and transfect the plasmids into cells in a 24-well plate. 24 h after transfection groups II and III were stimulated with 5 µg/µL Poly (I:C) for 12 h, and cell samples were collected for indirect immunofluorescence experiments. B Images such as those in panel A were subjected to image analysis (see Materials and methods), where > 30 cells for each sample were analyzed to determine Fn/c, the fluorescence in the nucleus relative to that in the cytoplasm. Results are means ± standard errors of the mean ( n ≥ 30). C DEFs were cultured in 6-well plates, grouped and transfected as described above for group A (transfection ratio of 3:2), 24 h after transfection groups 2 and 3 were stimulated with 20 µg/µL Poly(I:C) for 12 h, and cell samples were collected for Western blot analysis. D Gray value analysis was performed on the bands in C with ImageJ software to determine the ratio of Pn/c: Pn/c = Pn/Pc, Pn (relative protein level of IRF7 in the nucleus) = gray value of nuclear IRF7 protein/ Histone H3.1, Pc (relative protein level of IRF7 in the cytoplasm) = gray value of cytoplasmic IRF7 protein/β-tubulin. Where the data in each group were normalized relative to the control group. Values represent the mean (± SD) from three independent experiments. NS: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, compared with the control group.

Article Snippet: A mouse anti-Flag monoclonal antibody (Cat: M185-3 S) and a mouse anti-HA monoclonal antibody (Cat: M132-3) were purchased from Medical & Biological Laboratories Co., Ltd. A rabbit anti-duck IRF7 polyclonal antibody, a HRP-conjugated goat anti-mouse IgG heavy chain antibody (Cat: AS064), and a HRP-conjugated goat anti-mouse IgG light chain antibody (Cat: AS062) were prepared by ABclonal Technology Co., Ltd. A rabbit anti-beta (β)-actin antibody (Cat: 20536-1-AP) was obtained from Proteintech Co., Ltd. A rabbit anti-HA monoclonal antibody (Cat: AF2305), a mouse IgG antibody (Cat: A7028), and a HRP-conjugated goat anti-mouse IgG (Cat: A0216) were purchased from Beyotime Co., Ltd. A rabbit anti-Histone H3 (Cat: TA6358), a mouse anti-Myc (Cat: T62076M), and a mouse anti-beta (β)-tubulin monoclonal antibody (Cat: T63017-2) were purchased from Abmart Co., Ltd. A rabbit anti-VP3 antibody was prepared in our laboratory [ ].

Techniques: Translocation Assay, Transfection, Immunofluorescence, Fluorescence, Cell Culture, Western Blot, Software, Control

Journal: Veterinary Research

Article Title: DHAV 3CD targets IRF7 and RIG-I proteins to block the type I interferon upstream signaling pathway

doi: 10.1186/s13567-023-01134-4

Figure Lengend Snippet: A model of DHAV-1-mediated inhibition of IFNβ signaling pathway. After DHAV-1 infection, both viral proteins 3 C and 3CD can inhibit the production of IFNβ by interacting with IRF7 protein and inhibiting the expression of IRF7 protein, and the 3 C protein also inhibits the nuclear translocation of the IRF7 protein, blocking interferon signaling. In addition, the 3CD protein can interact with RIG-I to inhibit its expression, and can also interfere with the formation of a complex between RIG-I and MAVS to ultimately inhibit the RIG-I-mediated signaling pathway.

Article Snippet: A mouse anti-Flag monoclonal antibody (Cat: M185-3 S) and a mouse anti-HA monoclonal antibody (Cat: M132-3) were purchased from Medical & Biological Laboratories Co., Ltd. A rabbit anti-duck IRF7 polyclonal antibody, a HRP-conjugated goat anti-mouse IgG heavy chain antibody (Cat: AS064), and a HRP-conjugated goat anti-mouse IgG light chain antibody (Cat: AS062) were prepared by ABclonal Technology Co., Ltd. A rabbit anti-beta (β)-actin antibody (Cat: 20536-1-AP) was obtained from Proteintech Co., Ltd. A rabbit anti-HA monoclonal antibody (Cat: AF2305), a mouse IgG antibody (Cat: A7028), and a HRP-conjugated goat anti-mouse IgG (Cat: A0216) were purchased from Beyotime Co., Ltd. A rabbit anti-Histone H3 (Cat: TA6358), a mouse anti-Myc (Cat: T62076M), and a mouse anti-beta (β)-tubulin monoclonal antibody (Cat: T63017-2) were purchased from Abmart Co., Ltd. A rabbit anti-VP3 antibody was prepared in our laboratory [ ].

Techniques: Inhibition, Infection, Expressing, Translocation Assay, Blocking Assay